The practice of science communication is fundamentally changing. This requires science communication practitioners to continuously adapt their practice to an ever-changing ecosystem and highlights the importance of reflective practice for science communication. In this study, we supported 21 science communication practitioners in developing a reflective practice. Our study shows that reflective practice enabled practitioners in becoming aware of their own stance towards science or assumptions regarding audiences (single-loop learning), underlying and sometimes conflicting values or worldviews present in science communication situations (double-loop learning), and facilitated practitioners to adapt their practice accordingly. Triple-loop learning, allowing practitioners to fundamentally rethink and transform their mode of science communication, was less observed. We argue that reflective practice contributes to opening-up public conversations on science — including a conversation on underlying values, worldviews, and emotions, next to communicating scientific facts.