Public attitudes toward genetically modified organisms (GMOs) in Kenya remain mixed due to limited knowledge, policy gaps, and cultural factors. Despite the 2020 commercialisation of Bt cotton, perceptions of GM technologies are largely unfavourable. This study surveyed 416 respondents across 14 counties to assess awareness and knowledge levels of GMOs. Results showed 49% support GMOs, 27% are uncertain, and 24% oppose them. Misconceptions persist, with 49.3% citing size and 22.4% recognising labelling as a means of identifying GMOs. Awareness of GMO commercialisation was low (24%), though nearly half correctly identified Bt cotton as an approved GMO, and 32.7% unaware of any institution carrying out GM research. Education significantly influenced acceptance ($\upchi^2$ = 68.322, p 0.001). Radio was the most trusted information source (29.3%), and scientists were the most credible (46.4%). The findings underscore the need for targeted public communication strategies to address misinformation and enhance understanding of biosafety and biotechnology in Kenya.