Scientists are increasingly affected by harassment, especially on social media. While initial research highlights the detrimental consequences for affected scientists, the increased visibility of harassment through social media might also negatively affect public perceptions of scientists. Using a preregistered 2x2 between-subjects experiment (N = 1,246), this study shows that exposure to uncivil comments harassing female or male scientists negatively affects citizens’ trust in the attacked scientists but not trust in scientists in general or scientific information. Furthermore, some of the effects are moderated by gender and science-related populist attitudes.